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Saturday November 22, 2008

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Sporicidal Activity

Spore Tested	Source	Dilution	Independent test report
Aspergillus niger	University of Milan, Italy	1:100	51
Bacillus cereus	Laboratoire Simone, Belgium, 555 Protocol	1:100	30
	Lisbon University, AFNOR protocol	1:100	98
	Food Research Laboratory, Finland	1:100	40
Bacillus subtilis	Public Health Laboratory Service, UK	1:100	59
Bacillus subtilis	University of Milan, Italy	1:100	51, 99
Clostridium sporogenes	Laboratoire Simone, Belgium, 555 Protocol	1:100	30

Spore	Independent evaluation	Virkon S dilution	Starting Count	Contact Time (Mins) and log reductions
Spore	Independent evaluation	Virkon S dilution	Starting Count	1	5	10	15	20	30	45	60	120	180
Aspergillus niger	(Prof Dubini, University of Milan, Italy)	1%	1x106	G	G	G	-	G	NG	-	NG	-	-
		1%	1x105	G	G	G	-	NG	NG	-	NG	-	-
		1%	1x104	G	G	NG	-	NG	NG	-	NG	-	-
Bacillus cereus	(Lab Simone, Belgium, 5-5-5 protocol)	1%	6.0x105	-	Zero recovery	-	-	-	-	-	-	-	-
Bacillus cereus	(Lisbon University, AFNOR protocol)	1%	1x107	-	-	-	-	-	-	-	>5	-	-
Bacillus subtilis	(Dr Coates, Preston Public Health Laboratory Service, UK)	1%	3.2x107	-	-	-	-	-	-	-	3-5	>5	>5
		2%	3.2x107	-	-	-	2-3	-	>5	>5	>5	-	-
		3%	3.2x107	-	-	-	4-5	-	5	5	5	-	-
Bacillus subtilis	(Prof Dubini, University of Milan)	1%	1x106	G	G	G	-	G	G	-	NG	-	-
		1%	1x105	NG	NG	NG	-	NG	NG	-	NG	-	-
		1%	1x104	NG	NG	NG	-	NG	NG	-	NG	-	-
Clostridium sporogenes	(Lab Simone, Belgium, 5-5-5 protocol)	1%	2.9x107	-	Zero recovery	-	-	-	-	-	-	-	-

Numbers = log reductions recorded, G = Growth NG = No Growth, - = no measurement at this time point

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